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CHANGE-seq-BE reveals biological activity differences between nucleases and base editors To evaluate biochemical activity differences between base editors (ABE8e) and nucleases (Cas9), we additionally performed CHANGE-seq (with Cas9 nuclease) on gDNA from human primary T cells for the same five gRNAs ( B2M , CBLB , CD7 , CIITA and PDCD1 ) we previously evaluated with ABEs

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"Expression of bacterial genes in plant cells"

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