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The researches left the another foot unstimulated and found out that there was no such activity seen in the control foot

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Free Radical Biol Med (2015) 88(Part B):20511

Abstract: Pastore, A., Federici, G., Bertini, E., & Piemonte, F

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After TMB substrate solution is added, only those wells that contain Mouse FETUA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color