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To address this, we co-transfected MBP-tagged c-Jun with the DDD-Ube2e2-COP1 complex and performed tandem purification steps (Fig

The appearance of a pronucleus was used as the indicator of successful activation, suggesting that the oocyte had completed meiotic maturation and was capable of initiating embryonic development independently of fertilization

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However, in keeping with our other observations, melatonin-treated AA-challenged mice exhibited significantly improved airway function, with reduced hyperreactivity and restored dynamic compliance ( Figures 1O, P )