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The uptake and apoptosis test in 3D tumor spheroids To establish 3D tumor spheroids, 50 L of a 1% agarose gel solution (Aladdin) was added into each well of a 96-well plate

GHRP-6, as the structural ghrelin closest-analogue, has become the preferred research tool for probing these non-GH functions

VPA-exposed rats displayed abnormally high social investigation toward the familiar Stranger 1 compared to controls ( P 0.05 )

Researchers should avoid freeze-thaw cycles as these temperature transitions can denature the protein structure