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Nicotine (2 ng/mL cutoff), three nicotine metabolites [cotinine (5 ng/mL cutoff), 3-OH-cotinine (50 ng/mL cutoff), nornicotine (2 ng/mL cutoff)], and the tobacco metabolite anabasine (3 ng/mL cutoff)] were measured using quantitative liquid chromatography-tandem mass spectrometry (ARUP Laboratories, Salt Lake City, UT) and adjusted for urinary creatinine (see17 for further details on methodology)

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