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I was a latchkey kid once I convinced my mom that I didnt need a babysitter any more

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Two Versions, One Unforgettable Song Both Don Williams and Waylon Jennings brought something unique to Amanda. Williams version is tender and contemplative, with his gentle voice wrapping around the lyrics like a warm embrace

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RNA isolation and qRT-PCR Total RNA was extracted from cell lines with TRIzol Reagent (Invitrogen, CA, USA) following the manufacturers instructions, and was reverse-transcribed into cDNA, using M-MLV-RT and oligo(dT) primer (Invitrogen, CA, USA)