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Nunc T-25 EasYFLask tissue culture flasks (ThermoFisher Catalog #156,340, Waltham, MA) were coated for 2 h with Basal Medium (BEBM medium), collagen, bovine serum albumin, and fibronectin prior to subculturing

In particular, the health care system offers important opportunities to reduce smoking, especially for vulnerable populations, by implementing system changes to make tobacco dependence treatment a standard of care and by working with health insurers to cover evidence-based cessation treatments with minimal barriers and to promote their use ( 3 , 6 )

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This is evident as the program already restricts items like alcohol, cigarettes, pet food, paper products, and party favors

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Begin by removing any loose items, trash, and personal belongings from the car