Under normal physiological conditions, up to about 9098% of cellular glutathione is in the reduced GSH form, and a shift toward higher GSSG levels is widely used as an indicator of oxidative stress and impaired cellular health
The ability of p53 to bind to the GSL2 response element under stressed or unstressed conditions suggests that GSL2 can affect p53 function
Cells were subsequently blocked by 2% BSA in PBS-T (0.1% Tween 20 in PBS) for 1 h at room temperature, incubated with the primary antibody Anti-Rheb (Santa Cruz Biotechnology Cat# sc-271509, RRID: AB_10659102, 1:500) overnight at 4 C in blocking buffer, then washed with PBS-T three times and incubated with Alexa-555-conjugated secondary antibody (Invitrogen # A-31570, RRID:AB_2536180, 1: 1000) and DAPI (1 g/ml) in blocking buffer for 1 h at room temperature with protection from light
Food Secur
Wuertzm S, Reiser S
Moreover, there is substantial evidence that inflammatory processes play a key role in the relationship between exposure to chronic life stress and are linked to many diseases, including insulin insensitivity (69)