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Samples were normalized to total lung protein and used to assess the abundance of IL-6, IL-33, CCL20, Eotaxin-1 (DuoSet ELISA Kits, R&D Systems, Minneapolis, MN, USA), IL-4, IL-13 (eBioscience Kits, Thermo Fisher Scientific, Waltham, MA, USA), and MUC5AC (Novus Biologicals, Littleton, CO, USA) per manufacturer's instructions

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