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Code availability Not applicable Funding The authors extend their appreciation to the Deanship of Scientific Research at King Khalid University, Saudi Arabia, for funding this work through the Large Research Groups Program under grant number R.G.P2/274/44

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To investigate this putative lineage relationship, we targeted the POU2F3 and FOXI1 TF genes, required for tuft cell 19 and ionocyte specification, respectively 17,19 , using CRISPR/Cas9 KO in human tracheal basal cells ( n = 5 donors, Supplementary Fig

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