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Isolation of PBMC PBMC were immediately isolated from fresh whole blood by density gradient centrifugation according to the method of Boyun 50 using equal volume of blood and Ficoll (Histo-paque 1077)

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Chemical components in tobacco can interfere with the production, secretion, and levels of hormones, affecting the hypothalamus, thyroid, adrenal glands, and ovarian function [6]