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This is similar to Lpez et al

Other risk factors for oral cancer include: Chewing tobacco or other smokeless tobacco products Chewing betel nuts with or without added tobacco An unhealthy diet The human papillomavirus (HPV)

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Following the addition of 4 Laemmli sample buffer [250 mM Tris pH 6.8, 4% SDS, 45% glycerol, 715 mM mercaptoethanol, 50 mM DTT, 0.02% (w/v) bromophenol blue], the sample was boiled for 5 min, and centrifuged at 20,000 g for 30 min before immunoblotting

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These include: mouth blisters tooth or jaw changes heart palpitations or rapid heartbeat allergy symptoms People taking prescribed antihypertensives should review possible interactions with a doctor before starting nicotine gum