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All regions of the oral cavity, including the tongue, roof of the mouth, gums, and cheeks, are susceptible to cancer from tobacco use [1, 2]

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The following day, sections were rinsed with PBS and then incubated for 3 h at 2225 C with Cy3-conjugated anti-mouse (Jackson ImmunoResearch, 715-165-150) at 1:500 dilution in a solution of 1.5% BSA in PBS, respectively

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