Isolation of rat heart mitochondria Upon cervical dislocation of 912 weeks old male Wistar rats (Charles River, UK), heart mitochondria (RHM) were prepared by homogenization of their heart tissue in STEB buffer (250 mM sucrose, 5 mM Tris-HCl and 1 mM EGTA, pH 7.4) supplemented with 0.1% fatty acidfree BSA
In summary, the present studies add to our understanding of gut microbiota involvement in nutrient metabolism relevant to red meat ingestion, a Western diet, and CVD risks
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