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The SPR and recombinant-protein assays provide stronger evidence for a direct interaction than the earlier pathway studies

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Methotrexate also forms polyglutamates that inhibit other enzymes such as thymidylate synthase and aminoimidazole carboxamide ribonucleotide transformylase (AICART), resulting in the accumulation of adenosine, a potent anti-inflammatory mediator

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