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M1 macrophages release IL-23 and IL-1 that promote Th1 and Th17 responses implicated in airway neutrophilia and acute airway hyper-responsiveness

We then performed qPCR by using SYBR Green FastMix (Quanta Bioscience) on CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA)

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