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For immunohistochemical analysis, hind legs of E15.5 C57BL/6J mice were prepared, fixed in 4% paraformaldehyde phosphate buffer solution (Wako) overnight at 4C, and embedded in paraffin

While nicotine is addictive, the combusted tobacco is what causes negative health effects, such as cancer, emphysema, and heart disease

Of course, the easiest way would be to stop smoking, but hey, no judgement here

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Primary independent variables included cigarette smoking status (current smoker

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