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Activated Nrf2 orchestrates a multifaceted cytoprotective response through four principal mechanisms: scavenging ROS, downregulating NLRP3 expression, suppressing caspase-1 cleavage, and inducing NQO1 (Hennig et al., 2018

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Although each of the four enzymes can be identified by its reactivity with specific substrates, all of the enzymes are active to differing degrees in the conjugation of glutathione with p-nitrobenzyl chloride