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As noted in the peer-reviewed literature, bremelanotide is a melanocortin receptor agonist of interest for the study of central melanocortin signaling pathways (sciencedirect.com)

Gene expression: By taking biopsies of participants tongues

That sounds convenient and easy but there are some drawbacks

Thirst is generally a good guide, though some patients may need to drink even when not thirsty, especially during GI side effects

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Notably, the concentration of e-CAT in humans was higher than in other mammalian species, exceeding five to six times the levels in goat and sheep