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Trp53 fl/fl ) or intracolonic induction of colon tumours by submucosal injection of 100 M 4-hydroxytamoxifen (Sigma-Aldrich) in 810-week-old AKP mice (Villin-CreER T2

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Lysates were boiled at 95 C for 5 min, 20 g of total protein were subjected to separation by a 10% SDS gel, transferred onto nitrocellulose membranes (Amersham Biosciences) and blocked with 5% milk powder in PBS Tween (0.1%) for 1 h

Moving bodily fluids display significant disparities in velocities, often differing by several orders of magnitude

N., Swen, J

I completed a full 10-week cycle, took four weeks off, then started another round