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Instead, it exemplifies how endogenous peptides exhibiting multi-receptor activity were historically disadvantaged by a development paradigm that prioritized single-target selectivity over physiological relevance

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Twenty micrograms of each sample were then loaded onto 10% NuPAGE Bis-Tris gels (precast NuPAGE, Invitrogen), and transferred onto 0.45 m nitrocellulose membranes (Cytiva, Velizy-Villacoublay, France)